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multiphor ii electrophoresis  (Danaher Inc)


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    Danaher Inc multiphor ii electrophoresis
    Multiphor Ii Electrophoresis, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiphor+ii+system/pmc11366540-58-9-20
    Average 86 stars, based on 1 article reviews
    multiphor ii electrophoresis - by Bioz Stars, 2026-10
    86/100 stars

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    other:

    Article Title: Effects of human and porcine bile on the proteome of Helicobacter hepaticus
    Article Snippet: Isoelectric focusing was conducted using the Multiphor II system (Amersham Biosciences, Australia) at a maximum of 3500 V for 21 h until a total of 65 500 V-hour were achieved.

    Article Title: Alterations in the cardiac proteome of the spontaneously hypertensive rat induced by transgenic expression of CD36.
    Article Snippet: Fatty acid translocase (FAT/CD36) plays an important role in fatty acid uptake by different cell types and may also participate in regulation of calcium homeostasis and eicosanoid production.. CD36 deficiency or polymorphisms in the CD36 gene are linked to some physiological irregularities.. It is known that the expression of FAT/CD36 is aberrant in the spontaneously hypertensive rat (SHR), one of the most widely studied rat strains in cardiovascular research.

    Article Title: Identification of proteins in susceptible and resistant Brassica oleracea responsive to Xanthomonas campestris pv. campestris infection.
    Article Snippet: Cruciferous plants are important edible vegetables widely consumed around the world, including cabbage, cauli-flower and broccoli.. The main disease that affects crucifer plants is black rot, caused by Xanthomonas campestris pv. campestris (Xcc).. In order to better understand this specific plant-pathogen interaction, proteins responsive to Xcc infection in resistant (União) and susceptible (Kenzan) B. oleracea cultivars were investigated by 2-DE followed by mass spectrometry.

    Article Title: Crystallins Are Regulated Biomarkers for Monitoring Topical Therapy of Glaucomatous Optic Neuropathy
    Article Snippet: The rehydrated strips were focused on a Multiphor II system (Amersham Biosciences Europe) at approximately 80 kVh.

    Article Title: Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10days; comparison with animals exposed to morphine and subsequently nurtured for 20days in the absence of this drug.
    Article Snippet: Abbreviations: ABC, ammonium bicarbonate; ACN, ac CBB, Coomassie Brilliant Blue; CNS, central nervous syste methylphenylalanine-5-glycineol)-enkefalin]; DADLE enkefalin]; 2D-ELFO, two-dimensional gel electrophores δ-OR, δ-opioid receptor; DTT, dithiothreitol; EDTA, ethy ELFO, electrophoresis; FDR, false discovery rate; GPCR, G-p teins, heterotrimeric guanine nucleotide-binding regu cholamidopropyl)dimethylammonio]-1-propanesulfon high-performance liquid chromatography; IEF, isoelectric tor; LC–MS/MS, liquid chromatographymass spectrometr MALDI-TOF MS/MS, Matrix-assisted laser desorption/ioniz trometry; MMTS, methyl methane-thiosulfonate; μ-OR, μ chloride; Na, K-ATPase, sodium plus potassium, ouabai NOP-OR, nociceptin-orphanin receptor; PBS, phosphat membrane; PMSF, phenylmethylsulfonylfluoride; PNS, po dium dodecyl sulfate; SLB, sample loading buffer; w.w., w acid; TCEP, tris(2-carboxyethyl)phosphine; TEAB, tetraet trifluoroacetic acid.. ⁎ Corresponding author.. E-mail address: svobodap@biomed.cas.cz (P. Svoboda

    Electrofocusing:

    Article Title: Antitumour activity of Helix hemocyanin against bladder carcinoma permanent cell lines
    Article Snippet: 2-DE analysis of proteins in CAL-29 tumour cells after treatment with HlH After determination of the protein concentration of supernatants with the Protein Assay Kit (BioRad), approximately 250lg of protein was mixed with IPG rehydration buffer (8mol/L urea, 2% w/v CHAPS, 0.3% dithiothreitol (DTT), final volume of 360lL). .. The strips were allowed to rehydrate for 7 h and to focus (IEF) using a Multiphor II system (Amersham Biosciences) running the following program: 150 V (300), 150 V (1200), 300 V (300), 300 V (450), 3500 V (900), 3500 V (5400), 500 V (100), and held at 500 V. The temperature was kept at 18 C. After completion of the IEF program, the IPG strips were equilibrated in a 50 m mol/L TrisHCl solution, pH 8.8, containing 6mol/L urea, 30% glycerol, 2% SDS, and 1% DTT, for 10min, after which the solution was replaced with the same solution, except that DTT was exchanged by 5% iodoacetamide. ..

    SDS Page:

    Article Title: Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies.
    Article Snippet: Opioid addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations.. However, there is a lack of information about the changes at both the cellular and molecular levels occurring after cessation of drug administration.. The aim of our study was to determine alterations of both phosphoproteome and proteome in selected brain regions of the rats (brain cortex, hippocampus, striatum, and cerebellum) 3 months after cessation of 10-day morphine treatment.



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    Specification of the Relevant Instrumental Techniques for LF Characterization

    Journal: Journal of Agricultural and Food Chemistry

    Article Title: The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties

    doi: 10.1021/acs.jafc.3c06887

    Figure Lengend Snippet: Specification of the Relevant Instrumental Techniques for LF Characterization

    Article Snippet: , IEF , electromigration , Different pI values determined by IEF also might be a matter of the selected separation conditions, e.g. the type of carrier ampholyte (CA), pI marker, buffer composition, and separation time. The possibility of distinction of several LF fractions is based on their pI values. , Multiphor II apparatus (Amersham, Freiburg, Germany); precast gels Servalyt and Blank precotes (Serva, Germany); gel parameters, 126 × 125 × 0.3 mm; temperature, 5–8 °C; voltage, 2000 V; current, 6 mA; power, 12 W , ( , ) .

    Techniques: Isolation, Glycoproteomics, Staining, Mass Spectrometry, Concentration Assay, Spectrophotometry, Clinical Proteomics, Zeta Potential Analyzer, Marker, Spectroscopy, Software, Raman Spectroscopy, Functional Assay, Fluorescence, Sequencing, Single Particle, Microscopy, Chromatography, Solvent, Evaporation, Differential Scanning Calorimetry, Injection